Aeskulap-Stab
Introduction
Resolution and 
 focal depth
Principles of 
 luminance contrast
Modification of 
 lenses
Mirror objectives
Illuminating  
 apparatus
Material and  
 methods
Results
Further technical 
 developements
Discussion
Summary
Links
Contact
References
Image gallery
Introduction

In light microscopy, transparent specimens are usually examined by dark field, phase contrast and interference contrast light microscopy.

In dark field, specimens are illuminated by oblique light beams that come from the periphery of the illuminating apparatus. Therefore, some transparent objects, e.g. unstained native bacteria, are barely visible and fine structures inside them are oftenly not visible.

In phase contrast, the discernment of fine detail can be reduced by halo artifacts. The intensity of contrast, i.e. the difference in brightness between the background and specimen, is constant and not variable; it is determined by the specification of the phase ring within the phase contrast lens and dependent on the specific phase differences between the specimen and its surrounding medium.

Interference contrast images are free from halo artifacts, but their contrast may be lower than in corresponding phase contrast or dark field images, especially, when transparent specimens are examined in thin-layer preparations.

Recently, luminance contrast has been developed by the author as a new illumination technique characterized by several advantages in comparison with the usual examination methods suitable for transparent specimens. With the help of luminance contrast, such specimens can be examined in several variations of contrast effects, which are similar to dark field, phase and interference contrast. Therefore, these variants have been named luminance dark field, luminance phase contrast and luminance interference contrast. As opposed to common dark field imaging, internal structures within the specimen are also visible in high contrast. As opposed to common phase and interference contrast, the intensity of contrast is adjustable in tiny steps; and halo artifacts are either reduced or do not exist. The light path is completely different from common dark field, phase and interference contrast. In all variants of luminance contrast, the specimen appears as a self-luminous, flourescent object resulting in fundamental improvement in resolving power.

This new method has already been published (8) and applied for a patent.

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                               German version

Copyright: Joerg Piper, Bad Bertrich, Germany, 2007ight:
 

[Introduction]
[Resolution and focal depth]
[Principles of luminance contrast]
[Modification of lenses]
[Mirror objectives]
[Illuminating apparatus]
[Material and methods]
[Results]
[Further technical developements]
[Discussion]
[Summary]
[Links]
[Contact]
[References]
[Image gallery]